Semiquantitative Plate Assay for Determination of Cellulase Production by Trichoderma viride
نویسندگان
چکیده
منابع مشابه
Enhanced cellulase production by a mutant of Trichoderma viride.
A mutant strain that secretes twice as much cellulase as its parent was obtained by irradiating conidia of Trichoderma viride QM 6a with a linear accelerator.
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Feedlot waste contains essentially all the necessary nutrients for batch fermentation with the fungus Trichoderma viride. The organism utilizes two-thirds of the carbohydrate in feedlot waste while elaborating cellulase in quantities comparable to commercial preparations. Essentially odor-free, the fermented waste contains all of the original nitrogen but has 24% less organic matter.
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Microbial cellulases are the enzymes widely studied due to their enormous applications in biochemical industry. Among 12 fungal isolates isolated from mangrove plant debris and soil sample collected from Valanthakad Mangroves, Kerala, India, 3 of them were found to exhibit cellulolytic activity. Among them, the most potent isolate which exhibited maximum cellulolytic activity was identified as ...
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Cellulase-producing fungi Trichoderma viride were cultured and fermented on the solid-state wheat bran fermentation medium. The characteristics of its carboxymethyl cellulase (CMCase) in the condition of this solid-state fermentation were evaluated, and the optimum culture time, optimum pH and optimum temperature for CMCase activity of T. viride fermented in this solid state were 60h, 5.0 and 5...
متن کاملOptimization of Extracellular Cellulase Production by Trichoderma harzianum
ABSTRACT Background and Objectives: Cellulose is a major component of plant biomass, which can be converted into biofuels and valuable chemicals. The key step in utilization of this organic material is its hydrolysis into soluble sugars. This study evaluated cellulase production by Trichoderma harzianum under different pH values, temperatures and incubation...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1977
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.33.1.178-183.1977